LIQUID NITROGEN BIOLOGICAL CONTAINERS

LIQUID NITROGEN BIOLOGICAL CONTAINERS (KAIGEN and LEO)

I. Application Areas: frozen storage of fine-breed livestock semen (artificial insemination), cryopreservation of human organs, skin, blood, cells, and biological samples; also widely used in cryogenic surgery, industrial cold assembly, cold treatment of metal materials, cryogenic crushing, superconducting magnets, and other applications.

II. Product Structure: The container consists of an outer liner, an inner liner, a neck tube, insulation materials, a suction nozzle, a lifting cylinder, etc.

III. Production Materials and Processes

1. The outer liner and the inner liner are made of high-strength, corrosion-resistant aluminum alloy plates, which are sturdy and durable.

2. The cover is made of special plastic, which has the dual functions of reducing the evaporation of liquid nitrogen and fixing the lifting cylinder.

3. The neck tube is made of special fiberglass reinforced plastic, with high strength, low thermal conductivity, and a thermal conductivity coefficient that is only 2% of stainless steel's.

4. The insulation material is filled between the inner liner and the outer liner, and is manufactured by layered winding using advanced technology.

IV. Product Parameters

1. Water volume: 1.3 L to 100 L

2. Neck diameter: 30 mm, 35 mm, 50 mm, 80 mm, 125 mm, 130 mm, 210 mm, 216 mm

3. Outer diameter: 180 mm to 610 mm

4. Height: 360 mm to 1270 mm

V. Product Categories

(I) Storage type liquid nitrogen tank

Suitable for large-volume and long-term storage of liquid nitrogen and biological samples under static conditions, and users who need to frequently transfer and preserve samples manually.

KAIGEN product features:

1. Made of high-strength aviation aluminum;

2. Small and light, easy to carry;

3. Low evaporation rate of liquid nitrogen;

4. Large capacity;

5. Equipped with a protective cover to prevent the product from being bumped during use;

6. The bucket can be numbered for easy identification and independent storage and retrieval of samples;

7. Optional lock cover to protect the safety of stored samples;

8. High vacuum multi-layer insulation design.

(II) Transport type liquid nitrogen tank

Suitable for short-distance transportation.

KAIGEN product features:

1. Made of lightweight, high-strength aviation aluminum;

2. Contains multiple support structures, resistant to tipping;

3. Shockproof structure, suitable for long-distance transportation;

4. Low evaporation rate of liquid nitrogen;

5. The bucket can be numbered for easy identification and independent storage and retrieval of samples;

6. Equipped with a protective cover to prevent the product from being bumped during use;

7. Optional lock cover to protect the safety of stored samples;

8. High vacuum multi-layer insulation design.

(III) Laboratory liquid nitrogen tank

Suitable for laboratory use and can also meet the needs of users who use liquid nitrogen to perform deep freezing treatment on parts and components.

KAIGEN product features:

1. Made of lightweight, high-strength aviation aluminum;

2. Small size occupying less space;

3. Large diameter;

4. Large capacity;

5. Low liquid nitrogen consumption;

6. Equipped with a special lock cover to ensure sample safety;

7. Equipped with freezing racks and freezing boxes;

8. Equipped with transport casters for easy movement.

(IV) Liquid nitrogen tank for animal husbandry

It is widely used in the storage of frozen semen, vaccines, cells, microorganisms, and sampling, in animal husbandry, machinery manufacturing, electronics, metallurgy, energy, and other applications. Small liquid nitrogen tanks are suitable for users who manually transfer and preserve biological samples, especially for users in mountainous areas.

KAIGEN product features:

1. Made of lightweight, high-strength aviation aluminum;

2. Contains multiple layers of strongly insulation material with low volatility;

3. Features a numbered barrel position index;

4. Large capacity;

5. Multiple caliber options for easy access;

6. High vacuum multi-layer insulation design with low evaporation loss;

7. Equipped with a protective cover to prevent the product from being bumped during use;

8. Optional safety lock cover structure to prevent others from opening without authorization.

(V) Air transport liquid nitrogen tank

Suitable for transporting samples in deep low-temperature environments (gas-phase storage, temperature below -190°C), which can avoid the risk of liquid nitrogen release during transportation and is suitable for short-term air transportation such as airplanes.

KAIGEN product features:

1. Gas phase low temperature storage;

2. No liquid nitrogen overflow: There is a liquid nitrogen adsorbent inside, which can absorb and preserve liquid nitrogen. Even if the container is overturned, there will be no liquid overflow;

3. Liquid nitrogen filling is simple and fast;

4. Wheat straw and cryotube storage options;

5. Strong and durable aluminum structure;

6. Special stainless steel mesh screen to divide the storage space;

7. Lockable lid;

VI. Precautions For Use

1. The container can only be used to fill liquid nitrogen, not liquid chlorine, liquid oxygen, and other liquid gases.

2. The container must be strictly prevented from collision and falling during use to avoid damaging the high vacuum state of the tank.

3. It is forbidden to use storage tanks as transport tanks. Special transport tanks are required to transport liquid nitrogen.

4. It is forbidden to use sealed stoppers to close the tank mouth to prevent liquid nitrogen from continuing to evaporate, and form increased nitrogen pressure, causing accidents.

5. The vacuum exhaust port is an important part of the closed vacuum. It must be strictly protected and must not be hit, thrown, or twisted. Once this place is damaged, the container may become unusable.

6. Lifting operation: In order not to reduce the activity of the stored materials, the lifting time should be as fast as possible. After the lifting tube is inserted into the container, the cover should be quickly closed to reduce the consumption of liquid nitrogen. Be careful when lifting and lowering the lifting tube, and do not scratch the inner wall of the neck tube.

7. Liquid nitrogen should be replenished in time: Under normal circumstances, when the remaining liquid nitrogen is less than 1/3 of the full capacity, it should be replenished in time to avoid necrosis of stored items.

8. The workplace should be ventilated: Nitrogen is a colorless, tasteless, odorless, and non-toxic gas, but in the case of poor ventilation, the evaporated nitrogen will reduce the indoor oxygen content and pose a risk of suffocation to the human body. Therefore, indoor ventilation should be strengthened to keep the indoor air fresh.

9. Do not damage the neck tube: After long-term use of the container, the inner wall of the neck tube will sometimes frost and condense into ice. Normally, this kind of frost and ice will not constitute an obstacle to the operation of the lifting tube, so do not scrape it with a knife. If you accidentally scratch the inner wall of the neck tube, it may cause the neck tube to leak and lose vacuum, making the container difficult to repair. The correct method is to blow it with hot air.

10. Do not twist off the core plug: The core plug is made of foam plastic. During the use of the container, sometimes it is found that the core plug is frozen in the neck tube and cannot be taken out. At this time, do not twist it hard to avoid twisting off the core plug. The correct method is to blow it with hot air to melt the ice and then take out the core plug.

11. After the first injection of liquid nitrogen, it is found that there is sweating around the neck of the container, or a large amount of white frost is attached, which proves that the vacuum degree of the container has deteriorated and can no longer be used. It should be replaced immediately and contact the manufacturer.

12. Container washing and drying: During the use of the container, the bottom of the inner tank will continue to accumulate water, breed bacteria, corrode, and contaminate the low-temperature storage products. To prevent the above faults, the container should be cleaned 1-2 times a year. The washing steps are as follows:

(1) Remove the cylinder from the container and leave it for two days until the temperature in the container rises to about 0℃.

(2) Pour 40-50°C warm water into the container and then wipe it with a cloth.

(3) Rinse with clean water.

(4) Invert the container to dry it. The drying method can be natural air-drying or hot air drying. If hot air is used for drying, the temperature should be limited to 40-50°C, and avoid using hot air above 60°C.

VII. Troubleshooting